Part:BBa_K515102

J23100 promoter - PA2652

Description

Translational subunit with included optimised insulator and RBS BBa_K515002, its expression is under the control of the constitutive promoter BBa_J23100. Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H). Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.

Compatibility

Device has been tested in E. coli DH5α strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Unknown
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 710
  Illegal NgoMIV site found at 812
  Illegal AgeI site found at 118
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1019
  

This BioBrick has been sequence verified.

Background

Malate responding chemoreceptor originally found Pseudomonas aeruginosa PA01 is a codon optimised translational subunit. This subunit BBa_K515002 contains optimised insulator and RBS sequence, its expression is under the control of constitutive promoter BBa_J23100. Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H). Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.

Compatibility

Device has been tested in E. coli DH5α strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of E. coli.