Translational_Unit

Part:BBa_K515002

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)

PA2652

Description

This part is a component of the BioBrick BBa_K515102. This part includes an optimised insulator, an RBS and a coding sequence for the malate-responsive chemoreceptor PA2652 from Pseudomonas aeruginosa PA01. The insulator allows the strength of the RBS to remain the same even when the promoters for this BioBrick are changed using PCR, with the insulator acting as the annealing sequence for the primers. The translation initiation rate for the RBS sequence is 42010. This part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 984


This BioBrick is sequence verified.

The characterisation data for the full device can be found here.

[edit]
Categories
//cds/membrane/receptor
Parameters
chassisE. coli DH5α
device_typeChemotaxis receptor
ligandsMalate
originP. aeruginosa
resistanceChloramphenicol