Composite
Part:BBa_K608351
Designed by: Theodhor Tiko Group: iGEM11_Freiburg (2011-09-14)
(correct) temperature sensitive promoter
This is based on part (BBa_K098995)
Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor (BBa_K098997) CDS.
Usage and Biology
This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein (BBa_K098997) which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).
The temperature sensitive promoter functioning at 28-30°C |
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//promoter
//regulation/constitutive
//regulation/multiple
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//promoter
//regulation/constitutive
//regulation/multiple
None |