Composite

Part:BBa_K608351

Designed by: Theodhor Tiko   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 15:58, 21 September 2011 by UsernameV (Talk | contribs)

(correct) temperature sensitive promoter

This is based on part (BBa_K098995)


Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor CDS.


Usage and Biology

This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).


Freiburg11 Presentation1.jpg

The temperature sensitive promoter functioning at 28-30°C

Freiburg11 Presentation3.jpg The temperature sensitive promoter functioning at 42°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//promoter
//regulation/constitutive
//regulation/multiple
Parameters
None