Coding

Part:BBa_K539119

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-17)
Revision as of 08:12, 18 September 2011 by Bettyyang (Talk | contribs)

crtY (lycopene cyclase)

This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).

We found a point mutation of BBa_K118008(iGEM08_Edinburgh) at XbalI site and we corrected it successfully.(ACCTAGT->ACTAGT)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None