Part:BBa_K525405
Fusion Protein of S-Layer SbpA and mCitrine
Fusion Protein of S-Layer SbpA and mCitrine
S-layers (crystalline bacterial surface layer) are crystal-like layers consisting of multiple protein monomers and can be found in various (archae-)bacteria. They constitute the outermost part of the cell wall. Especially their ability for self-assembly into distinct geometries is of scientific interest. At phase boundaries, in solutions and on a variety of surfaces they form different lattice structures. The geometry and arrangement is determined by the C-terminal self assembly-domain, which is specific for each S-layer protein. The most common lattice geometries are oblique, square and hexagonal. By modifying the characteristics of the S-layer through combination with functional groups and protein domains as well as their defined position and orientation to eachother (determined by the S-layer geometry) it is possible to realize various practical applications ([http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full Sleytr et al., 2007]).
Usage and Biology
S-layer proteins can be used as scaffold for nanobiotechnological applications and devices by e.g. fusing the S-layer's self-assembly domain to other functional protein domains. It is possible to coat surfaces and liposomes with S-layers. A big advantage of S-layers: after expressing in E. coli and purification, the nanobiotechnological system is cell-free. This enhances the biological security of a device.
This fluorescent S-layer fusion protein is used to characterize purification methods and the S-layer's ability to self-assemble on surfaces. It is also possible to use the characteristic of mCitrine as a pH indicator ([http://pubs.acs.org/doi/abs/10.1021/bm901071b Kainz et al., 2010]).
Important parameters
Experiment | Characteristic | Result |
---|---|---|
Expression (E. coli) | Localisation | Inclusion body |
Compatibility | E. coli KRX and BL21(DE3) | |
Inductor for expression | IPTG, lactose | |
Purification | Molecular weight | ~ 130 kDa |
Excitation / emission | 515 / 529 nm | |
Immobilization behaviour | Saturation protein / bead ratio | 7 - 9 * 10-4 |
Immobilization time | ? |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 104
Illegal BglII site found at 221
Illegal XhoI site found at 1996 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 3913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 493
Illegal BsaI.rc site found at 622
//function/reporter/fluorescence
//proteindomain/internal
biology | S-layer |
chassis | E. coli |
emission | 529 nm |
excitation | 515 nm |