Coding
tetO array

Part:BBa_K606026

Designed by: Kévin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)
Revision as of 18:05, 12 September 2011 by Kyauy (Talk | contribs)

tetO Array

The tetO array composed by a 10kb tetO sequence, where YFP:tetR (BBa_K606025) can concentrate and increase the fluorescence sensibility. Origins of the tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2093
    Illegal BamHI site found at 95
    Illegal BamHI site found at 601
    Illegal BamHI site found at 1171
    Illegal BamHI site found at 1752
    Illegal BamHI site found at 1960
    Illegal BamHI site found at 2577
    Illegal BamHI site found at 2727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 117
    Illegal BsaI site found at 735
    Illegal BsaI site found at 793
    Illegal BsaI.rc site found at 428
    Illegal SapI site found at 1179
    Illegal SapI site found at 3528


[edit]
Categories
//dna/bioscaffold
Parameters
negative_regulatorsTetR
receiverYFP-TetR binding