Coding
YFP:tetR

Part:BBa_K606025

Designed by: Kévin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)

Usage and Biology

The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter
Parameters
negative_regulators