Coding
Cpn10

Part:BBa_K538000:Design

Designed by: Bas Stringer (Sequence by Paul van Dieken)   Group: iGEM11_Amsterdam   (2011-07-28)
Revision as of 12:35, 28 July 2011 by B.Stringer (Talk | contribs) (→‎References)

Cpn10 (O. antarctica)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


Design Notes

The protein's sequence was published by Ferrer et al. in 2004[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts]. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[1] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.

Source

De novo synthesis by GeneArt[http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html]

References

[1] Ferrer et al. Functional consequences of single:double ring transitions in chaperonins: life in the cold, Mol. Microbiol. 53 (1), 167-182 (2004)