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- ...ia coli</i>, that mediates resistance to chloramphenicol. (Shaw 1983) CAT covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, ren It is encoded by the <i>cat</i> gene which is commonly used by the iGEM community as part <a href=”ht58 KB (7,551 words) - 03:40, 22 October 2019
- ...TT CTG AAT GCG CCG TCA CCG GAA GCA GAT TTT GTG CTT CGT GAG GTG TGG GAC TTC CAT TCC GGA AAT TAT AGT TGT CTG TAT TTT GAG ACT GCC GCA CCA TTC GCC GGG GCC CCA2 KB (427 words) - 18:11, 18 April 2023
- ...TT CTG AAT GCG CCG TCA CCG GAA GCA GAT TTT GTG CTT CGT GAG GTG TGG GAC TTC CAT TCC GGA AAT TAT AGT TGT CTG TAT TTT GAG ACT GCC GCA CCA TTC GCC GGG GCC CCA2 KB (427 words) - 18:13, 18 April 2023
- ...cted to make a large difference in terms of expression as ''E. coli'' uses CAT ~62% of the time and CAC ~38% of the time, so neither codon is extremely ra10 KB (1,636 words) - 10:57, 19 October 2016
- ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t30 KB (4,474 words) - 11:37, 10 October 2018
- ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t29 KB (4,206 words) - 14:06, 9 October 2018
- ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t ...)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(mOrange-lacZ aphA3)). All strains are tagged with mOrange and show t31 KB (4,550 words) - 14:09, 9 October 2018
- ...me reaction, CATI, CATII and CATIII. The genomic analysis of the different CAT types shows that the boundaries between them are not completely clear, howe CAT is an enzyme originally identified in Escherichia coli that mediates resist52 KB (6,767 words) - 03:59, 22 October 2019
- ...me reaction, CATI, CATII and CATIII. The genomic analysis of the different CAT types shows that the boundaries between them are not completely clear, howe CAT is an enzyme originally identified in Escherichia coli that mediates resist50 KB (6,490 words) - 03:58, 22 October 2019
- ...me reaction, CATI, CATII and CATIII. The genomic analysis of the different CAT types shows that the boundaries between them are not completely clear, howe CAT is an enzyme originally identified in Escherichia coli that mediates resist49 KB (6,411 words) - 03:59, 22 October 2019
- ...olony PCR (1333 bp) was performed on the transformed colonies with primers cat-PF and Pb-PR. The positive colonies were transferred and plasmid was extrac5 KB (670 words) - 13:29, 12 October 2023
- ...CG GAT AGC CCG GAC TTG AGT GAT TTT GCG TCG CGC GGG GGA AAA TTA GTT ATC TAT CAT GGA GCC GCG GAT GCG GCA TTC TCG GCG CTG GAT ACA ATT AAG TAT TGG AAT GCG GTT3 KB (612 words) - 02:31, 10 October 2023
- Our CAT sequence information comes from NCBI and is codon optimized for its role in The sequence information of CAT comes from human chromosome 11, and the CAT gene used in this team was synthesized by Genscript Biotech Corporation401 B (55 words) - 21:25, 11 October 2023
- Our CAT sequence information comes from NCBI and is codon optimized for its role in The sequence information of CAT comes from human chromosome 11, and the CAT gene used in this team was synthesized by Genscript Biotech Corporation401 B (55 words) - 21:26, 11 October 2023
- | align="center" style="background:#f0f0f0;"|'''k<sub>cat</sub> × 10<sup>-2</sup> (s<sup>-1</sup>))''' | align="center" style="background:#f0f0f0;"|'''k<sub>cat</sub>/K<sub>m</sub> (mM<sup>-1</sup>⋅s<sup>-1</sup>))'''2 KB (310 words) - 16:35, 17 October 2018
- 5’ AAA CAT TTA CCA CCT CCT CCA CCT TAT AGT TT 3’ 5’ AAA CAT TTA CCA CCT CCT CCA CCT TAT AGT TT 3’9 KB (1,376 words) - 04:12, 28 September 2013
- GelE forward primer:5'-ATG CACCACCACCACCACCAC AAG GGA AAT AAA ATT TTA TAC CAT TTT A-3' <BR> Backbone reverse primer:5'-ATT TCC CTT GTG GTG GTG GTG GTG GTG CAT TAG TAT TTC TCC TCT TTC TCT AGT AGC TAG-3' <BR>2 KB (441 words) - 15:03, 4 October 2013
- ...of PP1. (A).</b> <i>E. coli</i> strain BL21 (DE3) harbouring pGEX6P-1 PP1 CAT gamma was grown to an OD 600nm of 0.4, after which expression of the GST-PP ...ege of Life Sciences, University of Dundee) for providing the pGEX6P-1 PP1 CAT gamma vector encoding a GST fusion to PP1. This vector was used to purify t14 KB (2,216 words) - 15:08, 26 October 2013
- ...er repression to occur. If there are point mutations within a copy of the cat cassette at this location, or a different mechanism provides chloramphenico4 KB (583 words) - 17:49, 1 November 2013
- ...ly - pKD46 carries the bla gene (ampicillin resistance), pKD78 carries the cat gene(chloramphenicol resistance), pKD119 carries the tet gene (tetracycline ...ly - pKD46 carries the bla gene (ampicillin resistance), pKD78 carries the cat gene(chloramphenicol resistance), pKD119 carries the tet gene (tetracycline2 KB (297 words) - 13:51, 12 October 2013