Part:BBa_K4730005:Design

Level 1 MHETase plasmid

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1175
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3121
  Illegal AgeI site found at 1010
  Illegal AgeI site found at 2341
  Illegal AgeI site found at 2458
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 992
  Illegal SapI.rc site found at 1291
  

Design Notes

Our original terminator degraded in the machine so we used the EF-rrnB.

Source

ATG CCT ATC ATC CGC TCT ACC ACG GCC TTG GCA GCC ATT GCG TCA GGG TTT TTT TTA ACT GGC GTA GAC CAT GCC GAC GCT GCC GTT GCC GGG GAC TTT CCA AAT CAA TTT TTA AGT TGC TTT GAC GCG GCG AAT TTA ACG GAA ATC GAG CTT CCG GCT GAT GTG CAG GGT TTT CGC TTA ATT GAG ATT GCG GAG CAC GCC GGA GAT AAG GGA ATG CCT GCG CAC TGC GAG ATC GTC GGG GCC ATC AAT GAC CGC ATC AGC CCG GTC GAT GGC CAA CAC TAT TCG ATT AAG TTT CGC CTG CGC TTG CCC CAA GAC TGG AAT GGG CGC TTC TAC ATG GAG GGT GGG GGA GGT TCA AAT GGC GTC CTG AAA GAC GCT ATG GGA CCT ACA GGA TTG AAT CAG GAG GAC TCC GCA CTT GAG CGC GGC TTT GCA GTA GTT ACC ACA GAC TCG GGA CAC GAT AAC GAT ACT AAT AGT GAC TCC AAT GCC TCG GGC CGT TCG GCC TTC GGT ATG GAT CCG CAA GCA CGT CTT GAT TTT GGT TAC ATG AGC TAT GAC ATT GTA ACG CGC GTG GGC AAG GCC ATC GTA GAG AAG TAC TAC GGA GCC GCG CCA GAG AAG TCG TAC TTT ATC GGA TGC AGC GAA GGT GGT CGC GAG GCC GCC CTT ATG ACC CAG CGC TAT CCA GAC TTG TAC GAC GGG GTA GTT GCT GGG GCA CCC GGA ATT CAC TTC TCT TAC AGT GCT GCT TAT GCT CCG TTT CTT TTA CGT ATT TTC GGT AAT TTA GCG GAA ACG CGC AAC CAA TCT GGC CCA GAC GGT ATC CCT TTA CTG AAC AAA TTA TAT AGT GAT AAC GAT GTA CAG TTA ATC GCA GAT GCC GTG GTT GGA GCA TGC GAC GCC TTG GAT GGG CTT GAG GAT CGT ATG TCA AAC AAT ATT GAA GCA TGC ACC ACG GTA ACA GTT TTG CCA CGT CTG CGC GCT CTG ACG TGC AGC GGA GCG AAA GAA GAG GGG TGT TTG TTG GAG GAT CAG ATC GAT GCG TTT GTG GCT GGC ATG GCC GGA CCA GTT ACA TCC GAC GGA ACT CGT CTG TAT CCT GGT CAT CCA TGG GAT CCC GGC ATC GGA GGT CGT ATT GGC GAC AGT GTG AAT GAC GGT TTT CGT TCT TGG TGG TTC GGC TCC TAT GAC TCC GAC CAA AAT AAT GCA CGT AAA GTG ACC CTT TCC ACT CCA CAG CAC GCC ATG CTG TGG CAA ACT CCG CCC GTA CCA TTG CGC CCG GAT GAG TAC GTA CGT TTC GAG ATG AAC TTT AAC ATC GAC GAG ACT CCT GCA CTG GCG TAC GCA ACT ACG GAC TTA TAT CCG GTC TCC TCC GCC GAG TTG GGT AAT GCG GAT AGC CCG GAC TTG AGT GAT TTT GCG TCG CGC GGG GGA AAA TTA GTT ATC TAT CAT GGA GCC GCG GAT GCG GCA TTC TCG GCG CTG GAT ACA ATT AAG TAT TGG AAT GCG GTT AAC GAA ACG GCG GAC GGC CAG GCG GCG GAT TTC GCT CGC TTA TTC ATT ATC CCC GGA ATG AAC CAC TGC CAG GGT GGG CCT GCT ACG GAT GAT GTA GAT TTA TTA ACG CCT TTA ATG GCG TGG GTT GAG GAC GAC CTG CCC ATC GAA CGC TTG GAG GCT ACC GTA TCG AAT CCT GAC TAC TTC GGT GGG AAG AAC TTG TCT CGC CCC TTA TGT CCT TAT CCA CTG TAT GCT GAG TAT GAT GGG GAG GGA GAC CCA AGT AGC GCC GAA AGC TTC ACC TGT GTC GCG AAT TGA