Generator

Part:BBa_K325909:ArabinosetoLight

Designed by: Theo Sanderson and Will Handley   Group: iGEM10_Cambridge   (2010-10-23)
Revision as of 17:15, 25 October 2010 by PM (Talk | contribs) (→‎Protocol)

Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
This page describes the light output of BBa K325909 as a fucntion of Arabinose concentration in the media. The characterisation has been made in different chassis. TOP 10 cells as well as 2 mutants in which expression of H-NS was repressed.

THIS PAGE IS CURRENTLY BEING UPDATED.

Data

Figure 1 - Light output of BBa_K325219 as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.
Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for BBa_K325219. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats.
Data Notes Date Uploaded
Media:BBa_K325909ArabinosetoLight.xls Raw data from experiment 21/10/2010

Protocol

  1. The protocol for

<math> P_{out} = \frac{P_{max}[3OC_6HSL]^n}{K^n+[3OC_6HSL]^n} </math>

</center> 1

Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227. </div> [http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176. [http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.