Composite
Part:BBa_K389016
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-09-28)
VirA/G reporter device mRFP
This BioBrick contains a complete VirA/G receptor system with an mRFP (BBa_E1010) under the control of a vir promoter as reporter gene.
Input: acetosyringone
Output: expression of mRFP
Usage and Biology
This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated virA, so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the vir promoter and its activity after inducing the system with acetosyringone.
Important parameters
Experiment | Characteristic | Value | ||
---|---|---|---|---|
Transfer Function | Maximum induction level | 2.6 fold | ||
Maximum induction level reached | 150 µM acetosyringone | |||
Hill coefficient | 1.67 | |||
Switch Point | 26.5 µM acetosyringone | |||
Doubling time / h | without plasmid | 1.98 | ||
carrying K389016 | 2.57 | |||
carrying K389016 with 150 µM acetosyringone | 2.77 | |||
carrying K389016 with 1000 µM acetosyringone | 3.01 | Conformation analysis | ratio ccc monomer / % | 91.2 |
ratio ccc dimer / % | 3.2 | |||
ratio oc forms / % | 5.6 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4260
Illegal AgeI site found at 4372 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768
[edit]
Categories
Parameters
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
None |