DNA
Ori

Part:BBa_K320000

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-17)
Revision as of 10:35, 24 October 2010 by Perrudet (Talk | contribs)

Asaia replication origin

This sequence was obtain by making a PCR form the GFP plasmid of a straight of Asaia containing this plasmid. This strain has been provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.


This origin of replication works is Asaia and E.Choli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal AgeI site found at 1320
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None