Part:BBa_K320000

Asaia replication origin


This biobrick was obtained by a PCR on the pHM2-GFP plasmid (See: [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensii, an Asian malarial mosquito vector]). The Asaia that contained this plasmid was provided to us by the team of Prof. Guido Favia (Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy).

Asaia is a gram negative bacterium, member of the family of acetic acid bacteria in the alpha-Proteobacteria. [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia] is naturally present in the gut of at least four mosquito species which are responsible for the transmission of Malaria (Anopheles stephensii, Anopheles gambiae, Aedes aegypti and Aedes albopictus).


This origin of replication works in Asaia as well as E. coli. No other bacteria were tested but [http://www.mobitec.com/int/products/bio/04_vector_sys/pbbr122.html the literature] shows that it works in many gram-negative bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1160
  Illegal AgeI site found at 1320
  
• 1000
  COMPATIBLE WITH RFC[1000]