Composite
GFP-XylE

Part:BBa_K316006

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-21)
Revision as of 14:20, 22 October 2010 by Ks907 (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K316006 short</partinfo> Constructed to be combined with promoter and terminator. The GFP <bbpart>BBa_E0040</bbpart> is linked to XylE <bbpart>BBa_J33204</bbpart> ...)

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N-terminus his tagged-GFP-XylE fusion protein

Constructed to be combined with promoter and terminator. The GFP BBa_E0040 is linked to XylE BBa_J33204 monomer subunit by a GGGSGGGS linker with the aim to render the enzyme inactive, via preventing tetramerization (it’s functional form).


Parts were assembled by PCR primer extension - exact methods our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 442
    Illegal NgoMIV site found at 614
    Illegal AgeI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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