Coding
Tse2

Part:BBa_K314200:Design

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-18)
Revision as of 01:28, 21 October 2010 by Hargem (Talk | contribs) (→‎Design Notes)

Toxin Tse2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 downstream from F2620 .

Source

Pseudomonas aeruginosa

References

Cell Host Microbe.�2010 Jan 21;7(1):25-37 PMID: 20114026