Coding
Tse2

Part:BBa_K314200:Design

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-18)

Toxin Tse2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 downstream from F2620, probably due to leaky expression of the Tse2 toxin from this promoter.

Source

Pseudomonas aeruginosa

References

Cell Host Microbe.2010 Jan 21;7(1):25-37 PMID: 20114026