Coding
virG

Part:BBa_K389002:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 14:49, 5 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

Mutated virG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • point mutations G56V and I77V so it works without rpoA gene from Agrobacterium tumefaciens in Escherichia coli (YC Jung et. al., 2004)
  • RBS (BBa_B0034-like) included
  • additional stop codon TAA added
  • synthesized by Mr. Gene, so:
    • optimated codon usage for E. coli
    • removal of illegal restriction sites

Source

synthetic

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.