Protein_Domain
Part:BBa_J70611:Design
Designed by: Joseph Lynch Group: Knight Lab (2010-07-20)
RFC12 RFP Internal Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 552
Illegal AgeI site found at 664 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR amplified off of BBa_J04450 with primers:
GTTTCTT C GAATTC GCGGCCGC T ACTAGT GCTTCCTCCGAAGACGTTATCAAAGAG, RFP-F
GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGCACCGGTGGAGTGACGAC, RFP-R