Coding
ECFP-RIFMO

Part:BBa_K5439003:Design

Designed by: Osvaldo Sanchez, Diego Cota Barocio   Group: iGEM24_TecMonterreyGDL   (2024-09-30)
Revision as of 23:07, 1 October 2024 by Osvaldosan21 (Talk | contribs)

With the DNA fragments purified from an agarose gel, we performed ligation at a molar ratio of 1:5 for vector and insert, as shown in Figure 3. The total vector concentration was 100 nanograms, whereas the reaction volume was 20 µL. Next, Table 2 displays the protocol followed for the reaction.

Table 2. DNA ligation conditions.
Reactive Quantity
T4 DNA Ligase Buffer (10X) 2 µL
Vector DNA 100 ng
Insert DNA 773.5 ng
Nuclease-free water To 20 µL
T4 DNA Ligase 1.5 µL