Part:BBa_K5439003:Design

No part name specified with partinfo tag.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1947
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 2178
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2833
  

Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids. There are no scars between each protein. Crystal structures of RifMo from Nocardia farcinica have been reported, providing valuable insights into its molecular architecture and potential mechanisms of action Liu et al. (2016).

Source

The original sequence was reported by Liu et al. (2016). Sequence can be obtained through GenBank (CP000946). The sequence for BBa_K1159302 was reported by Poëa-Guyon et al. (2013). The sequence for BBa_K1907000 was reported by Nagai et al. (2002).

References

[1]. Liu, L.-K., Abdelwahab, H., Martin Del Campo, J. S., Mehra-Chaudhary, R., Sobrado, P., & Tanner, J. J. (2016). The Structure of the Antibiotic Deactivating, N-hydroxylating Rifampicin Monooxygenase. Journal of Biological Chemistry, 291(41), 21553–21562. https://doi.org/10.1074/jbc.m116.745315

[2]. Poëa-Guyon, S., Pasquier, H., Mérola, F., Morel, N., & Erard, M. (2013). The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging. Analytical and bioanalytical chemistry, 405(12), 3983–3987. https://doi.org/10.1007/s00216-013-6860-y

[3]. Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., & Miyawaki, A. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nature Biotechnology, 20(1), 87–90. doi:10.1038/nbt0102-87