Part:BBa_K1132010

Tp901 integrase

The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.

The 180° switch permits to design a lot of regulation tools, such as logical gates that can be found here (https://parts.igem.org/Part:BBa_K1132001, https://parts.igem.org/Part:BBa_K1132002).

attB + attP + integrase → attR + attL + integrase

Improvement by iGEM Peking 2017

This part has been improved by iGEM Peking 2017.

Click here to get more information about Part:BBa_K2243000!

Contribution by ETH Zurich 2016

Group: ETH Zurich 2016

Author: Lukas Schmidheini

Summary: We cloned and characterised a codon optimised TP901-1 for E. coli, and sent it to the registry as a biobrick. Our biobrick can be found with a degradation tag Part:BBa_K2116010 and without any degradation tag Part:BBa_K2116035. The non-codon optimised version can be found here Part:BBa K1132010. Besides some favorable features, the codon optimized TP901-1 also supports full RFC[25] assembly and is available under the Tet promoter Part:BBa_R0040: TP90-1: Part:BBa_K2116064, TP901-1 with ssrA tag: Part:BBa_K2116062.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 28
  Illegal AgeI site found at 1486
  
• 1000
  COMPATIBLE WITH RFC[1000]