The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.
The 180° switch permits to design a lot of regulation tools, such as logical gates that can be found here (http://parts.igem.org/Part:BBa_K1132001, http://parts.igem.org/Part:BBa_K1132002).
Improvement by iGEM Peking 2017
This part has been improved by iGEM Peking 2017.
Contribution by ETH Zurich 2016
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 28
Illegal AgeI site found at 1486
- 1000COMPATIBLE WITH RFC