Coding

Part:BBa_K197030:Design

Designed by: John Wang   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 04:21, 22 October 2009 by Johnwang (Talk | contribs) (New page: __NOTOC__ <partinfo>GFP-LVA</partinfo> <partinfo>BBa_K197030 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source=== <pre> PCR mv022/mv014 on pBca1256-K112109 (1492bp, E...)

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Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

Source

PCR mv022/mv014 on pBca1256-K112109    (1492bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112111
-----------------------------------------------
mv022	Forward Biobricking of <ligase!
ccataGAATTCatgAGATCTattcttaaaattctgaacg
mv014	Reverse Biobricking of ligase!
cgttaGGATCCtcatagaccagttacctcatg

References