Part:BBa_K197030:Design
No part name specified with partinfo tag.
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 716
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 716
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 716
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 716
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Design Notes
This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]
This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]
The donor plasmid pBca1256-Bjh1857, which contains the gene GFP-LVA, underwent a Gateway Transfer reaction with the recipient plasmid pBca1254AK.
Source
Plasmid pBca1256-Bjh1857
References
Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64: 2240-2246.
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol Plant Microbe Interact. 13(11): 1243-1250.