Composite

Part:BBa_K5117040

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)
Revision as of 14:48, 1 October 2024 by Jesa98 (Talk | contribs)


PcotYZ-BsRBS-BhBglA-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
  • ribosome binding site of Bacillus subtilis (BBa_K5117000),
  • bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
    • addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117028),
  • cotY gene of Bacillus subtilis (BBa_K5117022),
  • bidirectional terminator B0014 (BBa_B0014).


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of BhBglA on the spore crust of B. subtilis (spore surface display)

Application: Degradation of cellobiose


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 764
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None