Composite
Part:BBa_K5117036
Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann Group: iGEM24_TU-Dresden (2024-09-28)
PcotYZ-BsRBS-BsEglS-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- eglS</li> gene of <i>Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i> addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024)
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1696
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]
</ul>
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellulose
Sequence and Features
Assembly Compatibility:
[edit]
Categories
Parameters
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