Part:BBa_K5143015

Venus : reporter Yellow Fluorescent Protein

Description

Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.

Venus Yellow Fluorescent Protein used as a transcriptional reporter.

Construction

The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (BBa_K5143009) in 5'. See the composite part here: BBa_K5143026

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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References

1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).

2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).