Composite

Part:BBa_K5143026

Designed by: Perrel Aymeric   Group: iGEM24_UnivLyon1-INSALyon   (2024-09-27)


Alphafactor-Venus, a secreted chromoprotein

Protein Description

Description

The Alphafactor-venus composite part was used in a larger composite part BBa_K5143027that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.

Venus fluorescence test
Figure 1: Promoter activation test by measuring the Venus fluorescence

Construction

Alphafactor-Venus is composed of the alphafactor V1 BBa_K5143009 and the venus chromoprotein BBa_K5143015.
Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (BBa_K4081994).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011).
2) https://pubmed.ncbi.nlm.nih.gov/25871405
3) https://parts.igem.org/Part:BBa_K5143015


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