Part:BBa_K5477008
XRE - Xenobiotic Response Element
XRE is Xenobiotic Response Element found in eukaryotic cell upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (AhR) which is responsible for signal transduction of pollutants (1) (2). XRE was used in our reporter module to drive the expression of NanoLuc under the presence of PAHs or PCB. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor system to detect PAHs and PCBs.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BamHI site found at 3 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
- 1000COMPATIBLE WITH RFC[1000]
References
1. Huang G, Elferink CJ. A novel nonconsensus xenobiotic response element capable of mediating aryl hydrocarbon receptor-dependent gene expression. Mol Pharmacol. 2012 Mar;81(3):338-47. doi: 10.1124/mol.111.075952. Epub 2011 Nov 23. PMID: 22113079; PMCID: PMC3286297.
2. Kuramoto, Nobuyuki & Baba, Katsuhiro & Gion, Keiko & Sugiyama, Chie & Taniura, Hideo & Yoneda, Yukio. (2003). Xenobiotic response element binding enriched in both nuclear and microsomal fractions of rat cerebellum. Journal of neurochemistry. 85. 264-73. 10.1046/j.1471-4159.2003.01679.x.
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