Coding

Part:BBa_K5115085

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 10:44, 29 September 2024 by Chenliyue (Talk | contribs)


F1v (FKBP with F36V mutation) without stop codon

contributed by Fudan iGEM 2023

Introduction

This part is FKBP with F36V mutation, which is a signal transducer. Binding with F1v, the target protein can get dimerized with B/B Homodimerizer in the culture solution.[1]

Usage and Biology

In BBa_K5115086(NixA-F1v) and BBa_K5115087(F1v-NixA), we link this part with BBa_K5115071(NixA) in two different ways to find the more efficient way of dimerizing the NixA protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ https://www.takarabio.com/documents/User%20Manual/iDimerize%20Inducible%20Homodimer%20System%20User%20Manual%20%28PT5178/iDimerize%20Inducible%20Homodimer%20System%20User%20Manual%20%28PT5178-1%29_111115.pdf?srsltid=AfmBOoqcK3gD-UsdZMD_JLop5oY-5L8W9a82H6GI4TmsKvN2gEBf37iH
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Categories
Parameters
None