Other
MiddleLink

Part:BBa_K243005:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 13:08, 21 October 2009 by Timo (Talk | contribs) (→‎Source)

Middle Linker ( Gly-Gly-Ser-Gly)x2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Freiburg09 Middlelipart.png
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file

Source

Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.

References