Other
MiddleLink

Part:BBa_K243005:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)

Middle Linker ( Gly-Gly-Ser-Gly)x2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.
The sequence has no cleavage site for proteases.
Freiburg09 Middlelipart.png
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file

Source

Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.

References