Reporter

Part:BBa_K4770013

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-11)
Revision as of 04:55, 12 October 2023 by Andreacami (Talk | contribs) (→‎How can this part be used)


mCherry_Gene

Description and Biology

Red Fluorescent Protein mCherry is a constitutively monomeric red fluorescent tetrameric protein original from Discosoma sp. (sea anemones). Generally used as a reporter or tag for the visualization of genes as intracellular prove, it presents improved brightness and photostability over similar photoproteins.

How can this part be used

In AlgaGenix, mCherry sequence has been used for MoClo assembly as a B3-B5 piece to create a reporter cassette that can be adapted for position 6 in Level Ms.

Source of this part

Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018).

Design considerations

MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan. Sequence contains an intron (CrRbcS2 intron 1) for an intron-mediated enhancement (IME).


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 349
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None