Part:BBa_K5115026

csoS2

Introduction

CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium Halothiobacillus neapolitanus, CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in E. coli.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 133
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 291
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 799
  Illegal AgeI site found at 1750
  Illegal AgeI site found at 2431
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 191
  

References

[1] Li, T., Jiang, Q., Huang, J., Aitchison, C. M., Huang, F., Yang, M., Dykes, G. F., He, H. L., Wang, Q., Sprick, R. S., Cooper, A. I., & Liu, L. N. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nature communications, 11(1), 5448. https://doi.org/10.1038/s41467-020-19280-0

[2] Oltrogge, L. M., Chaijarasphong, T., Chen, A. W., Bolin, E. R., Marqusee, S., & Savage, D. F. (2020). Multivalent interactions between CsoS2 and Rubisco mediate α-carboxysome formation. Nature structural & molecular biology, 27(3), 281–287. https://doi.org/10.1038/s41594-020-0387-7