Composite

Part:BBa_K5205014

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-SDG   (2024-09-24)
Revision as of 04:16, 24 September 2024 by Registry (Talk | contribs)

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J23100-Urease gene cluster

This is a complete expression cassette consisting of a strong constitutive promoter BBa_J23100, a urease gene cluster from Sporosarcina pasteurii DSM33 BBa_K5205012, and a T7 terminator BBa_K731721.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3438
    Illegal BamHI site found at 5155
    Illegal XhoI site found at 4192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4450
    Illegal BsaI.rc site found at 496
    Illegal BsaI.rc site found at 2224
    Illegal BsaI.rc site found at 4739


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