Composite

Part:BBa_K5205012

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-SDG   (2024-09-24)
Revision as of 03:44, 24 September 2024 by Registry (Talk | contribs)

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The urease gene cluster from Sporosarcina pasteurii DSM33

This is the complete urease gene cluster of S. pasteurii DSM33 consisting of ureA-ureB-ureC-ureE-ureF-ureG-ureD (Pei et al., 2023). ureA BBa_K5205005, ureB BBa_K5205006, and ureC BBa_K5205007 encode for structure subunits of the ureases, and ureE BBa_K5205008, ureF BBa_K5205009, ureG BBa_K5205010, and ureD BBa_K5205011 encode for assisting proteins (Ciurli et al., 2002; Moersdorf et al., 1994; Remaut et al., 2001; Zambelli et al., 2005). The urease enzyme complex is crucial for catalyzing the hydrolysis of urea into ammonia and carbon dioxide, a key step in the process of microbially induced calcite precipitation (MICP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3403
    Illegal BamHI site found at 5120
    Illegal XhoI site found at 4157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4415
    Illegal BsaI.rc site found at 461
    Illegal BsaI.rc site found at 2189
    Illegal BsaI.rc site found at 4704


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