Composite

Part:BBa_K5317022:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)
Revision as of 14:43, 22 September 2024 by Vanessa09 (Talk | contribs) (→‎References)


3xCre3xAP1-miniCMV-miRFP670


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1132
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 739
    Illegal NgoMIV site found at 774
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was optimised for human cell lines.


Source

This sequence was obtained from Eurofins.

References

Hai, T. W., Liu, F., Coukos, W. J., & Green, M. R. (1989). Transcription factor ATF cDNA clones: An extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. Genes & Development, 3(12b), 2083–2090. https://doi.org/10.1101/gad.3.12b.2083

Miller, M., Donat, S., Rakette, S., Stehle, T., Kouwen, T. R. H. M., Diks, S. H., Dreisbach, A., Reilman, E., Gronau, K., Becher, D., Peppelenbosch, M. P., Van Dijl, J. M., & Ohlsen, K. (2010). Staphylococcal PknB as the First Prokaryotic Representative of the Proline-Directed Kinases. PLoS ONE, 5(2), e9057. https://doi.org/10.1371/journal.pone.0009057