Composite

Part:BBa_K5317022:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)


3xCre3xAP1-miniCMV-miRFP670


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1132
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 739
    Illegal NgoMIV site found at 774
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was optimised for human cell lines.


Source

This sequence was obtained from Eurofins.