Composite
Part:BBa_K5317022:Design
Designed by: Vanessa Bruhn Group: iGEM24_Hannover (2024-09-22)
3xCre3xAP1-miniCMV-miRFP670
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1132
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1132
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 788
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1132
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1132
Illegal NgoMIV site found at 451
Illegal NgoMIV site found at 739
Illegal NgoMIV site found at 774 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was optimised for human cell lines.
Source
This sequence was obtained from Eurofins.