Part:BBa_K5317002
miRFP670
PknB is a eukaryote-like serine/threonine kinase in Staphylococcus aureus that consists of an N-terminal cytosolic kinase domain, a central transmembrane domain and three C-terminal extracellular PASTA domains.2 When this extracellular domain recognises beta-lactam compounds, it transmits the signal by autophosphorylating the N-terminal kinase domain, thereby activating downstream signalling cascades.3 In S. aureus, this mechanism normally enables early recognition and adaptation to antibiotic stress.4 In our project, we aim to introduce PknB into HEK cells, together with three proteins that can be phosphorylated by PknB. This targeted phosphorylation increases DNA binding activity 1 and is expected to induce the expression of a specific gene, which generates a fluorescent signal.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 763
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 763
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 419
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 763
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 763
Illegal NgoMIV site found at 82
Illegal NgoMIV site found at 370
Illegal NgoMIV site found at 405 - 1000COMPATIBLE WITH RFC[1000]
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