Part:BBa_K5049004
Xylanase-GCW61
Xylanases are enzymes that degrade xylan, a major component of plant cell walls, into simpler sugars. As feed additives, they play a crucial role in breaking down complex polysaccharides in animal diets, particularly for non-ruminants like poultry and swine. This enzymatic action enhances nutrient availability and digestion, leading to improved feed efficiency, growth performance, and overall health of the animals2. Moreover, the supplementation of xylanase in animal feed can significantly reduce feed costs and environmental impact by increasing nutrient absorption and decreasing nutrient excretion.
GCW61, one of the glycosylphosphatidylinositol-modified cell wall proteins (GPI-CWPs) in Pichia pastoris, plays a crucial role in maintaining normal morphology in yeast cells. This anchoring enable enzyme anchoring on the cell surface and performing functionality throughout feed processing, ensuring consistent performance in agricultural applications.
The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris and for xylanase from Streptomyces thermovulgaris. This composite part was constructed by the basic parts of <a href="https://parts.igem.org/Part:BBa_K5049003">BBa_K5049003</a> and <a href="https://parts.igem.org/Part:BBa_K5049001">BBa_K5049001</a>
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 529
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 856
Illegal AgeI site found at 844
Illegal AgeI site found at 1039 - 1000COMPATIBLE WITH RFC[1000]
| None |