Coding

Part:BBa_K5049001

Designed by: Wei Lai   Group: iGEM24_Mingdao   (2024-08-29)


GS-GCW61 (GCW61 anchor protein with a GS linker)

GCW61, one of the glycosylphosphatidylinositol-modified cell wall proteins (GPI-CWPs) in Pichia pastoris, plays a crucial role in maintaining normal morphology in yeast cells. This anchoring enable enzyme anchoring on the cell surface and performing functionality throughout feed processing, ensuring consistent performance in agricultural applications.

The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.The GS-GCW61 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None