Regulatory
Part:BBa_K5267006:Design
Designed by: Pengyu Zheng Group: iGEM24_NUDT-CHINA (2024-08-17)
6xCRE-Pmin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 118
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
You should consider the connection between the upstream and downstream sequence. The design was designed to account for the need to continuously accumulate the number of CRE copies, so two consecutive different restriction sites were added before the first CRE
Source
Homo Sapiens