Primer
Part:BBa_K4615000
Designed by: Xinyi (Marry) Xuan Group: iGEM23_Toronto (2023-10-12)
No part name specified with partinfo tag.
This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 27
Illegal PstI site found at 45 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 27
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |