Primer

Part:BBa_K4615000

Designed by: Xinyi (Marry) Xuan   Group: iGEM23_Toronto   (2023-10-12)


Forward primer to insert 20 bp of sgRNA of tpiA into pTargetF

This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 27
    Illegal PstI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 27
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 27
    Illegal XbaI site found at 33
    Illegal PstI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None