Composite
Part:BBa_K4759204:Design
Designed by: Jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-07)
T7-RBS4-SUMO-linker-OleP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 295
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 295
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 295
Illegal BglII site found at 184 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 295
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 295
Illegal AgeI site found at 420
Illegal AgeI site found at 518 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Add a solubilization-promoting tag at the N-terminal of the P450 enzyme
Source
company