Composite

Part:BBa_K4759204:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS4-SUMO-linker-OleP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
    Illegal BglII site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
    Illegal AgeI site found at 420
    Illegal AgeI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add a solubilization-promoting tag at the N-terminal of the P450 enzyme


Source

The solubilization-promoting tag SUMO was purchased from company

References