Coding
Part:BBa_K4825002:Design
Designed by: Chen Simiao Group: iGEM23_GreatBay-SCIE (2023-10-11)
ERG20ww
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 222
Illegal BamHI site found at 1059 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
Design Notes
Geranyl pyrophosphate(GPP) is precursor of pinene, which is our desired product. Therefore a higher yield of GPP is desirable, while the basic level of FPP is crucial for yeast survival. A method is designed to increase production of GPP while keeping FPP at a low level. It is to introduce 2 mutation F96W and N127W in ERG20, creating ERG20ww. The mutation in ERG20ww enables the reduction in FPP synthesis(from GPP), while GPP synthesis is not influenced. As a result, less GPP will be transformed to FPP, and a higher yield of pinene could be achieved.
Source
Saccharomyces cerevisiae