Coding

Part:BBa_K4825002:Design

Designed by: Chen Simiao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 11:44, 11 October 2023 by Registry (Talk | contribs)

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ERG20ww


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 1059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694


Design Notes

Geranyl pyrophosphate(GPP) is precursor of pinene, which is our desired product. Therefore a higher yield of GPP is desirable, while the basic level of FPP is crucial for yeast survival. A method is designed to increase production of GPP while keeping FPP at a low level. It is to introduce 2 mutation F96W and N127W in ERG20, creating ERG20ww. The mutation in ERG20ww enables the reduction in FPP synthesis(from GPP), while GPP synthesis is not influenced. As a result, less GPP will be transformed to FPP, and a higher yield of pinene could be achieved.


Source

Saccharomyces cerevisiae

References